18458 Ttll1 Status: Cancelled
| Gene | Ttll1 |
| Chromosome | 15 |
| Deletion Start | 83,505,359 |
| Deletion End | 83,484,095 |
| Deletion Size | 21,265 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGCTCAGTGCTTGGAACCAG |
| TUP | | | AACTAAGGAGCCTCCAAAGTGTTGGCG |
| TUR | | | AGCCACAAGCCTCTTCCATCAC |
| TDF | | | TGACCAGCAGCGAGAAGAAG |
| TDP | | | CCCAGCTTGGACCTTCCCACAGTC |
| TDR | | | TTAGCAGGGACTGGGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | TAGGGACTGGGCATTTATGG |
| SD | CAGCTTCATCAGGGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
