18493 Adh5 Status: ES cell colonies screened / QC no positives
| Gene | Adh5 |
| Chromosome | 3 |
| Deletion Start | 138,455,126 |
| Deletion End | 138,443,185 |
| Deletion Size | 11,942 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CGCGGGACGGAAGAACTTG |
| TUP | | | CGCCCGGGTTGCAGGATCAGATTT |
| TUR | | | AGGTCGAAGGAAGGGCCTAAC |
| TDF | | | GGCCTGTGAGGTGGATCAG |
| TDP | | | AGGTGCCTCCTGCCAAGCCTGAT |
| TDR | | | CATGCCTGTGGAGGTCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GAAGGCGTGGCTTGTTCTAC |
| SD | CCCTCTTGGACACCTGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
