18500 Zfp760 Status: Cancelled
| Gene | Zfp760 |
| Chromosome | 17 |
| Deletion Start | 21,724,217 |
| Deletion End | 21,719,111 |
| Deletion Size | 5,107 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGACCATGCCATGAAACTGATG |
| TUP | | | TGTCTGTGAGTAGCTGCAGATTGTAGTCTG |
| TUR | | | TGCCAAGTGCCAACAAACAAAG |
| TDF | | | CAAATGTGAGGAATGTGACAAATCC |
| TDP | | | ACCCACTGCTCAAGTCTTAAAGCTCATCA |
| TDR | | | AAGGTTTGACAACCAATAAAAGGAC |
PCR Primers
| Primer | Sequence |
| SU | TGACTTGGGTAGAATGGCAC |
| SD | TCCTTTACGCATTTGGTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
