18519 Trmu Status: Cancelled
| Gene | Trmu |
| Chromosome | 15 |
| Deletion Start | 85,897,259 |
| Deletion End | 85,879,380 |
| Deletion Size | 17,880 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TCCTTCGTTGGATGACAAACTTC |
| TUP | | | TCCGAACAGCTACCAGTTCTTCCTGAC |
| TUR | | | GGACCCATGAGGCTACATCTAC |
| TDF | | | ACACTGGATTGCGGAGGAG |
| TDP | | | CACCTGCTGCCTTGGTCAGAGACA |
| TDR | | | GGTGGCGGAATCGGAAATG |
PCR Primers
| Primer | Sequence |
| SU | AGCGAGTGGTGGCTAAGAAG |
| SD | AACACTGGCTTCCACCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
