| Gene | Zfp324 |
| Chromosome | 7 |
| Deletion Start | 12,971,634 |
| Deletion End | 12,966,207 |
| Deletion Size | 5,428 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGGAACTTTGTTGTAGGCACTTG |
| TUP | | | TGAGTCATCTAATGTGGACACTGGGAGC |
| TUR | | | TGGCTCAGCGGTTAAGAGTATG |
| TDF | | | TCTGCCGCAACTCACATC |
| TDP | | | TATTCAGCACGAACGCACGCACA |
| TDR | | | CAGAGGGAGCAGGCATAGG |
PCR Primers
| Primer | Sequence |
| SU | GAGCAAATAAGGATAGTACTCG |
| SD | CTGGTCCAAATCTCCTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18535D-A3 | VGB6 | C57BL/6NTac | |
| 18535D-D1 | VGB6 | C57BL/6NTac | |
| 18535D-D3 | VGB6 | C57BL/6NTac | |
| 18535D-D9 | VGB6 | C57BL/6NTac | |
| 18535D-D11 | VGB6 | C57BL/6NTac | |
| 18535D-F3 | VGB6 | C57BL/6NTac | |
| 18535C-B2 | VGB6 | C57BL/6NTac | |
