18570 Cacna1b Status: Cancelled
| Gene | Cacna1b |
| Chromosome | 2 |
| Deletion Start | 24,763,044 |
| Deletion End | 24,732,200 |
| Deletion Size | 30,845 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CCGCTCGCTCTTCGTCTTC |
| TUP | | | AGCGAGGACAACGTCGTCCGCAAA |
| TUR | | | GCCATTCGGTGATGCGCTTAG |
| TDF | | | GCTCACATGGGCATTCAC |
| TDP | | | TGTTAAGCGCTCCTCCCTTGATGC |
| TDR | | | TGGAGTCAGAGAACAGCAGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGACTGACGCGGATTGG |
| SD | GATCTCTGTTCATCTGTCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
