18578 Sesn1 Status: ES cell colonies screened / QC no positives
| Gene | Sesn1 |
| Chromosome | 10 |
| Deletion Start | 41,907,377 |
| Deletion End | 41,887,497 |
| Deletion Size | 19,881 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGGAGGTGTTGGAACAGAATAAGG |
| TUP | | | ACTGCTGTGCCCTGCCGGT |
| TUR | | | GCTCTGCTGCTCTGACTTG |
| TDF | | | TGAGAAGGTGCGTGCTTGTG |
| TDP | | | CTGCCCATTGTCATCCGCAGGCT |
| TDR | | | CGTTGAGATCCTGAGTGGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TAGGTGACGTTGCTGACTGC |
| SD | ACGTCCCAGTCTTTACGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
