| Gene | Lingo1 |
| Chromosome | 9 |
| Deletion Start | 56,621,300 |
| Deletion End | 56,619,416 |
| Deletion Size | 1,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ACATCTCCCATCGAGCCTTC |
| TUP | | | AGCGGCCTCAACAGCCTGGAACA |
| TUR | | | GATTGCATTTCTCCAGCGTCAG |
| TDF | | | ACCAGCTATCCTTTGGCTCTCAC |
| TDP | | | CCGCAAGCACTTGGTCTCGGC |
| TDR | | | AGCCGCCCATTGCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGTAGAGCACCCAGGAC |
| SD | ACTGAGTTGGAAGAAGGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18625A-D3 | VGB6 | C57BL/6NTac | |
| 18625A-F2 | VGB6 | C57BL/6NTac | |
| 18625A-F7 | VGB6 | C57BL/6NTac | |
| 18625A-G11 | VGB6 | C57BL/6NTac | |
| 18625A-H4 | VGB6 | C57BL/6NTac | |
