18639 Ppp1r3c Status: Cancelled
| Gene | Ppp1r3c |
| Chromosome | 19 |
| Deletion Start | 36,736,563 |
| Deletion End | 36,733,410 |
| Deletion Size | 3,154 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGGAGACTCCCAGGTTGATG |
| TUP | | | TGAGTTGAATCGTCCCGGAGGCTTT |
| TUR | | | TGACTCACTAAGGCAGTTTCTAC |
| TDF | | | CAGCCCTCAACCGACTAC |
| TDP | | | AAGTTTCCGGGACCGCTTTCAGAAGA |
| TDR | | | CGGTCACCGTCCGATCTTG |
PCR Primers
| Primer | Sequence |
| SU | CCAGGGCTCAGGGACTCTAC |
| SD | ACACCCTTACCACAGCGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
