18659 Tmeff2 Status: Cancelled
| Gene | Tmeff2 |
| Chromosome | 1 |
| Deletion Start | 50,943,220 |
| Deletion End | 50,927,951 |
| Deletion Size | 15,270 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TCCCACCTCAATCCTGAGAAAC |
| TUP | | | ATTGAGAACTGCTTCAGCTAAGGCTGGGC |
| TUR | | | CCCACACCTGTACTTTGTTCC |
| TDF | | | GGCAGGAAGAAGCACGGTAAG |
| TDP | | | AACAGTGCAGGTGGTGTTCCTGTGCC |
| TDR | | | GTAGTGGAGCAGACACTGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGGTTTCTGTCTCCTGCTCC |
| SD | CATGTCGACAACTTTGTATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
