18678 Rspo4 Status: Cancelled
| Gene | Rspo4 |
| Chromosome | 2 |
| Deletion Start | 151,873,426 |
| Deletion End | 151,843,077 |
| Deletion Size | 30,350 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGGTATTTGTCTGGTGCCTGATG |
| TUP | | | CCTGGACTGTTGACTCAGGGTCGGAA |
| TUR | | | CCCTCCCAGTAGTGCTTCC |
| TDF | | | CTCCCTGAACTGGACCTGTG |
| TDP | | | TCTTGCAGAAAGAAATCCCAGGCAGAA |
| TDR | | | CGCTCCAACTTCCTGTCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TGACGCCCTCACTCGACC |
| SD | ACTGGCCAGGTGAATGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
