18715 1500011H22Rik Status: Cancelled
| Gene | 1500011H22Rik |
| Chromosome | 5 |
| Deletion Start | 122,370,557 |
| Deletion End | 122,367,353 |
| Deletion Size | 3,205 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCACATCCTTTCCCATCTTG |
| TUP | | | AGGCCTGCAGCTCATCTTTATTGCACTGA |
| TUR | | | CAGCACAAACATCTGGAATCAACAC |
| TDF | | | GTGTACCTCCATCTGGTTTCTTG |
| TDP | | | TCAGGTCTTAGGAGAAGTGAGAGCATCTTC |
| TDR | | | GCACACCTGGAACACAAATCAC |
PCR Primers
| Primer | Sequence |
| SU | GCGTGTGCAGCTGTCTCTG |
| SD | GAGACTACCCTGCGTTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
