18741 Ankrd36 Status: Cancelled
| Gene | Ankrd36 |
| Chromosome | 11 |
| Deletion Start | 5,596,775 |
| Deletion End | 5,569,930 |
| Deletion Size | 26,846 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CACTGGTGGCCTTCACATC |
| TUP | | | AAGAGACCATCAAAGCCTTGGAGCTTGC |
| TUR | | | TCCTGAACTAGGGACCACATTG |
| TDF | | | TGCCGATTTAAGGCAACACAATG |
| TDP | | | CCCGGCATCTTTCAGTCTATGGCTTGA |
| TDR | | | CACTCTTGGATGTCTCCCTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTTGATGCTTGAAGGCACAG |
| SD | GCCTCGAGACTCTCTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
