18745 Aplf Status: ES cell colonies screened / QC no positives
Gene | Aplf |
Chromosome | 6 |
Deletion Start | 87,672,019 |
Deletion End | 87,646,019 |
Deletion Size | 26,001 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | | | CGCTGCTGGGAGTAAGTGTG |
TUP | | | CGTGGGCAGCCAGAGCTTGCTC |
TUR | | | CCCAAGCTCAGGAGACAAGAG |
TDF | | | CACAAATGAAGAGGGCGAGAG |
TDP | | | TGTGCACGTGTCCAAAGCAAGTCA |
TDR | | | GCAGAGCTAGACTCAGGATGG |
PCR Primers
Primer | Sequence |
SU | TTGCCTTCTGGGATTGGTAG |
SD | AATTAAGATACTAAGTAAGCAGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |