18820 Eif4g1 Status: ES cell colonies screened / QC one positive
| Gene | Eif4g1 |
| Chromosome | 16 |
| Deletion Start | 20,692,449 |
| Deletion End | 20,673,905 |
| Deletion Size | 18,545 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGTGTTTAGCACGCCTCAAG |
| TUP | | | CGACACAAATGAACACGCCTTCTCAGCC |
| TUR | | | TCCCACCCAAGCCTACTCAAG |
| TDF | | | GGAGGGATCTCCGGGTGTAG |
| TDP | | | TGCTAACGCTTTGGCTAGTCACCTAATTGA |
| TDR | | | AGCAGGTCTGCAAGCCACAAG |
PCR Primers
| Primer | Sequence |
| SU | GCCGCACTGTGGACTTATTC |
| SD | ACGGCAGTCCCAAGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
