18846 Gcc2 Status: Cancelled
| Gene | Gcc2 |
| Chromosome | 10 |
| Deletion Start | 58,280,692 |
| Deletion End | 58,255,569 |
| Deletion Size | 25,124 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCAGGAAGAAGCAAGGAAC |
| TUP | | | ATCCTGTTGGCTCCAGCTCCAGA |
| TUR | | | GCACAGTGCTGTAAGAACAACC |
| TDF | | | CTAATGTTGGTTGTGTTATGGGTTG |
| TDP | | | TTTATTTTTAGGAACATGTCAGTAC |
| TDR | | | TGAAATTGGAGTTGGAGTTCTTCG |
PCR Primers
| Primer | Sequence |
| SU | CGTGTGCTCAAACCTGTCAC |
| SD | TGAGCTTAGGTTCTTCTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
