18899 Mettl15 Status: Cancelled
| Gene | Mettl15 |
| Chromosome | 2 |
| Deletion Start | 109,274,655 |
| Deletion End | 109,274,392 |
| Deletion Size | 264 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GTCCCCACACAATCCACACTG |
| TUP | | | TGGCACTGCATACTGAATCTAAAGCTGTGG |
| TUR | | | CATGGCTTCCAACTCTTTCTCTTC |
| TDF | | | TGTGGAAGGGACAGCTCTG |
| TDP | | | TGCGGCCCTCAGAAGGTCTACA |
| TDR | | | GGTGCCAAGCAATGAACAAC |
PCR Primers
| Primer | Sequence |
| SU | ACATTGGATGAGCTTGGGTC |
| SD | GGACCCTCTGGAATTGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
