18925 Necab1 Status: ES cell colonies screened / QC no positives
| Gene | Necab1 |
| Chromosome | 4 |
| Deletion Start | 15,148,913 |
| Deletion End | 15,140,229 |
| Deletion Size | 8,685 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCTCTACCCTGCAACTGTCC |
| TUP | | | AGGGCATGTCCATCTTCCTCGACGTAA |
| TUR | | | AATGCGCCCACCTAACTACTTC |
| TDF | | | TCTGGGTAGGAAGCAGGTG |
| TDP | | | ACACTTCCTTCACTGAGGAGACATCGC |
| TDR | | | CTTTGTAAAGGGCACGAGTATTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTTCTTCCCACTCATGG |
| SD | AAGTAACAGCTCACATATGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
