18935 Nsmaf Status: Cancelled
| Gene | Nsmaf |
| Chromosome | 4 |
| Deletion Start | 6,454,040 |
| Deletion End | 6,424,885 |
| Deletion Size | 29,156 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGATGCGGGTTTGTCTGTGG |
| TUP | | | CCGGGATCCGACGGTCACGTTG |
| TUR | | | GCTGCACCTGCATTTCCTG |
| TDF | | | AGCTGCTGCACAGACACAGAAAG |
| TDP | | | CAGCCGTGAATCCAGGCTCTCCTG |
| TDR | | | GCTGGCAAAGTAAGATGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGAAATTTGGGACTAAACATCC |
| SD | AAACACATTCATCCAGTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
