18970   R3hdml     Status: ES cell colonies screened / QC positives

GeneR3hdml
Chromosome2
Deletion Start163,328,191
Deletion End163,318,231
Deletion Size9,961
Genome Build37
MGI IDMGI:3650937
NCBI Gene ID100043899
BAC ID130O6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTGGAGTATATGGTGAGTCCCAAAC
TUPCCCTGTGCACCATCCAGCCAA
TURCCCAGACCACAGTGAACAATC
TDFATCGGAGAGGCACCCTACAAG
TDPCCGGGAAGCCATGTTCTGCCTGTC
TDRTGTTGGATTTGAGCCCAGAGAAG

PCR Primers

PrimerSequence
SUTGGTAAAGAAGGCATTTGGC
SDAGCTGGAGATAACCAGCCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18970A-A2VGB6C57BL/6NTac
18970A-B11VGB6C57BL/6NTac
18970A-C8VGB6C57BL/6NTac
18970A-D8VGB6C57BL/6NTac
18970A-F3VGB6C57BL/6NTac
18970A-H3VGB6C57BL/6NTac