18975 Rassf4 Status: Cancelled
| Gene | Rassf4 |
| Chromosome | 6 |
| Deletion Start | 116,661,901 |
| Deletion End | 116,638,139 |
| Deletion Size | 23,763 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGAGCAACCTTTGGGAATCAC |
| TUP | | | TCCCTTCGTCCACCATGTGGGTCT |
| TUR | | | TGAGCAGGTAAAGGCACTTACTG |
| TDF | | | TCCGATAAAGCAAGCACAGAG |
| TDP | | | TCTGCTGGATAGAACCTCAGCCTAGTCC |
| TDR | | | TCCCACTGGGCTCAAACTTC |
PCR Primers
| Primer | Sequence |
| SU | CCATCAGCACACACCTGTC |
| SD | GCAATGAGAAGCAAGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
