18977 Rbm18 Status: Cancelled
| Gene | Rbm18 |
| Chromosome | 2 |
| Deletion Start | 36,134,244 |
| Deletion End | 36,117,801 |
| Deletion Size | 16,444 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGGACTCTTTGCCACTTG |
| TUP | | | TCAACTCTGTTCCCAGCATTTCTGGAGA |
| TUR | | | ATGCCTCAGGTTGGCTCTAC |
| TDF | | | CCTACGTGGTGGCTCATAACTG |
| TDP | | | TCTGGGACTCCAGTTCTAGGGAACC |
| TDR | | | TCCCAGAGTCTAGTAGAGTGCATC |
PCR Primers
| Primer | Sequence |
| SU | ACACAAAGAAAGCAGATGGTG |
| SD | TCTCACTTTCCACCCTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
