18994   S100a1     Status: ES cell colonies screened / QC positives

GeneS100a1
Chromosome3
Deletion Start90,316,055
Deletion End90,315,094
Deletion Size962
Genome Build37
MGI IDMGI:1338917
NCBI Gene ID20193
BAC ID461C14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGAACTGAAAGACCTGCTACAAAC
TUPTGAACTTTCTGGCTTCCTGGATGTAAGCA
TURCCACCCTGTACCTCAATCCTG
TDFGGGAAGTGGACTTCAAGGAGTATG
TDPTGCTGGTGGCTGCTCTCACAGT
TDRTGGTCTCCCAGAAGAAGTTGTTAC

PCR Primers

PrimerSequence
SUCAGAGGAGATCGTAGCCCAC
SDTCCTTGAGAAGCCAATGACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18994A-D12VGB6C57BL/6NTac
18994A-H10VGB6C57BL/6NTac