19016 Smc1a Status: ES cell colonies screened / QC no positives
| Gene | Smc1a |
| Chromosome | X |
| Deletion Start | 152,061,844 |
| Deletion End | 152,016,535 |
| Deletion Size | 45,310 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCAACCCGAGACATTGTGAAC |
| TUP | | | CCTGAAATGTTCCTTGATCCTCTTGGTGGG |
| TUR | | | CCGAACTCCGCCAACTGAC |
| TDF | | | TGAAGCCTGGAAGGGAAGAC |
| TDP | | | CCCAAGTTGGGACTGGGTTTCAAGGA |
| TDR | | | AGCTAGGGCACAATCAGGAATG |
PCR Primers
| Primer | Sequence |
| SU | TGCTGGGTAACTACGTAG |
| SD | TTACAAACCGAGCCCATTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
