19059 Ube3c Status: Cancelled
| Gene | Ube3c |
| Chromosome | 5 |
| Deletion Start | 29,602,344 |
| Deletion End | 29,569,518 |
| Deletion Size | 32,827 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GACATACCCGCTCCTCACAAAG |
| TUP | | | CTTGGAGTGAGTGCCTTGCCCTCA |
| TUR | | | CAGGCACGAGGGACTCTTC |
| TDF | | | GCTACCTGCTTCACCCACTG |
| TDP | | | ACAGGCTGTCCTGATTCCACCAGTG |
| TDR | | | GTCTCCTCGTTATCGTCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | AGCACCGTATTATCGATGGC |
| SD | CAAGGTTACTTCCTCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
