19132 Ano10 Status: Cancelled
| Gene | Ano10 |
| Chromosome | 9 |
| Deletion Start | 122,275,646 |
| Deletion End | 122,251,150 |
| Deletion Size | 24,497 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGGGCTTTGGGTGAGCAGATAG |
| TUP | | | CCCTAGGATACACAGAGCCACTCGCC |
| TUR | | | TCCAATCGACACCACTGACTTC |
| TDF | | | CGTGGTCACTAACTGTGCTC |
| TDP | | | TGGAATGTCACCACAAGTGAATGCAGTC |
| TDR | | | ACGGCCACCACAATCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGTCACCATTTCAGTGACATTTAG |
| SD | GACCAGGCGTAAAGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
