19263 Rpl37 Status: ES cell colonies screened / QC no positives
| Gene | Rpl37 |
| Chromosome | 15 |
| Deletion Start | 5,118,702 |
| Deletion End | 5,116,685 |
| Deletion Size | 2,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAAGCGCAAGAGGAAGTGTAAG |
| TUP | | | TACTGTAATCCGCAGGCGGGCTT |
| TUR | | | GTTGTTGCGTGCCAAATGTG |
| TDF | | | GCACAGACATGGATTCCGTGAG |
| TDP | | | AACAACGCCGAAACCCAAGAGGGC |
| TDR | | | GAAGAACTGGATGCTGCGACAG |
PCR Primers
| Primer | Sequence |
| SU | CAGAAACCCTCTCCAACCAG |
| SD | TCTTAAGACACTGTAGGCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
