| Gene | Gga1 |
| Chromosome | 15 |
| Deletion Start | 78,893,896 |
| Deletion End | 78,877,578 |
| Deletion Size | 16,319 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TCTGTGCAGGGTGGTGTG |
| TUP | | | CGACATGGTCAGCATCTTTGAAGCCA |
| TUR | | | TCAACCTTCCAGTTCAACTTCC |
| TDF | | | GGAGCAGTAGGTAGCAGTAGGTAAC |
| TDP | | | TGGCTGACTGACTTAGAAACTGTGACCA |
| TDR | | | AAGGAGGACAGGGAAGACTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCGCCCGGAGCTGCGAG |
| SD | CACACACCAAGTGCACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19350B-D11 | VGB6 | C57BL/6NTac | |
| 19350B-E1 | VGB6 | C57BL/6NTac | |
| 19350B-E9 | VGB6 | C57BL/6NTac | |
| 19350B-F11 | VGB6 | C57BL/6NTac | |
| 19350B-H1 | VGB6 | C57BL/6NTac | |
| 19350B-H8 | VGB6 | C57BL/6NTac | |
