19439 Rai12 Status: Cancelled
| Gene | Rai12 |
| Chromosome | 11 |
| Deletion Start | 69,980,256 |
| Deletion End | 69,968,332 |
| Deletion Size | 11,925 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGTGGGAAGGGCGTAGTC |
| TUP | | | TCCTGAAGGCTCTTATCAAGAAATCTGCAC |
| TUR | | | CGGGAAGGAGAGGAGAAACTG |
| TDF | | | GACAGACAAATGGCAGAGGAAG |
| TDP | | | TGGGCTCAGAACCACCCTGCT |
| TDR | | | ACAAAGGACAAGGGACAAGGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGACCTGGAGAGAAGCCAG |
| SD | TACCAGTGGTGTCTCCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
