19701   9530068E07Rik     Status: ES cell colonies screened / QC positives

Gene9530068E07Rik
Chromosome11
Deletion Start52,407,151
Deletion End52,396,471
Deletion Size10,681
Genome Build38
MGI IDMGI:2654705
NCBI Gene ID213673
BAC ID107D18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTAGGGACAGCGACCCAGTG
TUPTGGGACTGAATTGATTGACTGTTCCAGAGT
TURGACCTCAGAGATGGAATCCACAAAC
TDFGGGCCTGAGAAAGGACAGAATG
TDPTTACTGCTGTCAGAATGTCTTCCAGCC
TDRTCACTGGCTCACCGACAC

PCR Primers

PrimerSequence
SUTGACAACGATAAACCACAGAGC
SDGAATAAAGGACCGGAAACAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
19701A-C10VGB6C57BL/6NTac
19701A-D3VGB6C57BL/6NTac
19701A-E8VGB6C57BL/6NTac
19701A-G6VGB6C57BL/6NTac
19701A-G11VGB6C57BL/6NTac
19701A-H11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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