| Gene | Cd274 |
| Chromosome | 19 |
| Deletion Start | 29,385,421 |
| Deletion End | 29,372,471 |
| Deletion Size | 12,951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTGGCTTCTACCTGGTCTCTG |
| TUP | | | TTTGAACTTGGGTCCATGGGCTACACA |
| TUR | | | GGATGGAGTGATTGCCCAGTAAG |
| TDF | | | AAGGCCAGGCAGAACTAAAGTG |
| TDP | | | TTTCGCTAACTTGGGCAGACCCAA |
| TDR | | | CGGTTCCTCAGGGTGACTCAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCACTTGGTTCTACATGGC |
| SD | CATGCCCAAAGTGAGAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19754A-B10 | VGB6 | C57BL/6NTac | |
